Please note that since the NERCE program will end in February 2014, our ability to support new requests for services is limited.

Biomolecule Production Laboratory

The NERCE Biomolecule Production Core Laboratory is a core service laboratory with capability for expression and purification of recombinant proteins using bacterial and baculoviral expression systems, and preparation of bacterial carbohydrate components. It supports all New England investigators conducting research on NIAID priority pathogens that requires purified proteins or carbohydrates.

Resources and Services available

The Laboratory currently supports:

• Projects conducted at the NERCE Proteomics and Small Molecule Screening Cores that require purified proteins

• Vaccine development efforts that require large quantities of bacterial carbohydrate components for in vitro biologic assays and animal studies

• Investigators who must scale up expression of target proteins to expand their research

The laboratory is equipped with four 5L Applikon bioreactor systems with automatic temperature, dissolved oxygen, and pH control. Proteins and carbohydrates are purified using a column chromatography system with both UV/VIS and RI detectors. Carbohydrates are analyzed using a Dionex HPLC system. Protein purity is established using standard methods that include gel electrophoresis and western blot analysis. All procedures are conducted by core laboratory staff.

Application: Investigators are expected to provide data demonstrating efficacy of production and purification methods, as well as strains or purified recombinant plasmid preparations where applicable. A request is initiated by submission of a completed Core D Request Form to:

Biomolecule Production Core Facility
Armenise D2, Room 333
200 Longwood Avenue
Boston, MA 02115
biomol_prod@hms.harvard.edu

Download the Biomolecule Production Request Form.

Fees: There are no charges for projects focused on NIAID priority pathogens and agents of emerging infectious disease.

Contact:

Robin Ross, Ph.D. Assistant Director of Biomolecule Production Laboratory Robin.ross@channing.harvard.edu 617-432-5525 (laboratory) 617-432-5507 (office) 617-432-5508 (fax)