Please note that since the NERCE program will end in February 2014, our ability to support new requests for services is limited.
Live-cell Imaging Core
Live-cell imaging, using Spinning Disc technology is a powerful method. It enables investigators to observe cellular trafficking events with a high degree of spatial and temporal resolution, including visualization of individual bacteria or viral particles engaged in the process of infecting eukaryotic cells. A quantitative description of bacterial and viral invasion mechanisms can be obtained through such approaches. NERCE has provided funding to support the use of live-cell, Spinning Disc imaging for any New England investigator studying NIAID priority pathogens and agents of emerging infectious disease. This resource is located at the Immune Disease Institute on the Harvard Medical School campus in Boston, MA.
Resources and Services Available
• CSU-X1 and CSU-22 Spinning Disc confocal units with optical upgrades that increase light throughput two-fold over the manufactures specifications
• Hardware based, LED controlled auto-focus for long-term time-lapse acquisition
• Fully motorized, computer-controlled inverted microscopes
• Fully enclosed environmental chambers
• Heated stage plates for temperature deviation intolerance
• CO2 with humidity control
• Piezo ‘Z’ stage controllers for extremely fast, accurate and repeatable 3D image acquisition
• Computerized spherical aberration correction for imaging live or fixed cells and tissue samples
• Simultaneous acquisition of two fluorochromes (TIRF only)
• Accepts 1x3” slides, 35–50mm Petri dishes and glass bottom multi-well plates
• Widefield filers DAPI, CFP, GFP, YFP, RFP, Far Red
• LASERs 405, 473, 488, 561, 640 and 660nm
• 2D Time-Lapse
• 3D Acquisition and Display
• 4D Acquisition
• Spinning Disk Confocal
• FRAP and Photo-activation
• Object Counting (Automated)
• Particle Tracking
• Simultaneous Dual Probe Acquisition (TIRF only)
• Widefield Acquisition
• Cell Adhesion
• Cellular and Protein kinetics
• Membrane Traffic
• Gene Expression
• Axon Growth and Path Finding
• Cell Physiology
• Protein Translocation Across Membranes
The imaging core maintains state-of-the-art imaging equipment and provides extensive training on both the practical and theoretical aspects of the technology. Each user receives a comprehensive 52 page protocol covering system startup, maintenance, acquisition properties, system shutdown/troubleshooting and appendix that details the system components for your publication's methods section.
Training is provided as two, 2-hour sessions. In the first, while the user observes and follows along with the written protocol, the instructor sets up the system, alters component properties specifically for the user's experiment, and explains the function of each component in the system. During the second session, the user operates the system independently under the supervision of an instructor who will offer advice and answer questions.
Each system is encompassed by darkroom curtains with a comfortable 8x8’ of working space. An incubation chamber and additional space for cell work are available, including a fully operational tissue culture room if required.
Application For Use Investigators interested in working with the Live-Cell Imaging core laboratory should complete the Imaging Core Request Form and send it to Gerald Beltz at the e-mail address below.
Gerald A. Beltz, Ph.D.
Associate Director of Research
Download the Imaging Core Request Form.
Fees: There are no fees for use of the live-cell imaging core for research related to NIAID priority pathogens and agents of emerging infectious disease.
||Tomas Kirchhausen, Ph.D.
Senior Imaging Specialist
617 713-8885 (office)
Immune Disease Institute
Warren Alpert Building, Rm 133D
200 Longwood Avenue
Boston, MA 02115
||Gerald A. Beltz, Ph.D.
Associate Director of Research